A record crowd of 67,684 supporters attended the event at Twickenham last season, but officials say they are expecting up to 75,000 fans as the new season gets underway next weekend.
Tickets are valid for the two matches next Saturday, with London Irish playing defeated Premiership Rugby finalists Saracens at 2pm and London Wasps taking on Harlequins at 4.30pm.
Club matches will now have attracted over 150,000 supporters to Twickenham for the last two Premiership Rugby matches at the famous stadium.
Tickets are still available, however due to stadium regulations they will not be available on the day.
New Premiership Rugby title sponsors Aviva signed a four-year Ã‚Â£20 million deal earlier this summer to take over from Diageo Great Britain under the Guinness brand.
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