The Londoners take a 1-0 lead into Tuesday night’s Champions League semi-final second leg thanks to Didier Drogba’s goal at Stamford Bridge last week.
But Di Matteo admits it will be a huge challenge for his side to keep a clean sheet and wants the Blues to go all out to try and snatch what would be a vital away goal.
“Ultimately Barcelona will create chances against any team and we will have to try to score a goal but we will also try to limit the chances they create against us,” he said.
“It is too difficult to play for a goalless draw. To score would give us a greater advantage but we all know how difficult it is going to be.”
Di Matteo’s side managed to keep out Pep Guardiola’s holders last Wednesday before holding Arsenal to a goalless draw at Emirates Stadium at the weekend.
And the interim Chelsea boss has been thrilled with his side’s defensive stability since he took over from André Villas-Boas in early March.
“I always talk about the collective performance that helps us defend as a team and every individual has to do their job within it but I am very pleased with the way our defenders have been playing lately,” he added.
“We have defended very well and we have been impressed with Gary [Cahill] since he joined”
• The teams have met 11 times in Uefa competition; Chelsea have four wins to Barcelona’s three, with four draws.
• Barcelona have won three of the clubs’ four two-legged contests in the Champions League knockout phase.
• Roberto Di Matteo and Pep Guardiola were on opposing sides for the first of these Champions League contests in 1999-00.
• Barcelona are unbeaten in 15 European home fixtures dating back to October 2009, with 13 wins and two draws. They have scored 18 goals in five home games this season.
MORE: Man United latest news
MORE: Arsenal latest news
MORE: Chelsea latest news
MORE: Liverpool latest news