The Italy international was hauled off early in the second half after an ineffective display at The Etihad as Sir Alex Ferguson’s men ran out 3-2 winners after a thrilling derby.
Balotelli looked less-than-impressed with his manager’s decision to replace him with Carlos Tevez as he trudged straight down the tunnel in the 51st minute.
And Mancini told the striker that he “cannot continue” to play like he did on Sunday, as Manchester City slipped six points behind Premier League leaders United.
“I love Mario but I think it’s important for him to start thinking about his job,” said Mancini. “He can’t continue to play like today.
“When a player has this quality you can’t understand he will throw it out the window. This is incredible.
“There aren’t words I can say. It’s a bad feeling. When you lose a derby in the final second, it’s hard.”
Get 8/1 enhanced odds on Tottenham to win or 11/1 enhanced odds on Liverpool to win at White Hart Lane on Saturday afternoon. Click here to sign up now. T&Cs apply.
MORE: Latest Man Utd news
MORE: Latest Arsenal news
MORE: The latest football news