Former Liverpool defender Martin Kelly scored a first-half winner to end Tottenham’s participation in the cup competition.
Spurs are still going strong in Premier League and Mauricio Pochettino’s side are two points behind leaders Leicester City.
The north London side secured a 1-1 draw with Fiorentina in the first leg of their Europa League last-32 clash in Florence on Thursday.
And Kane issued a rallying cry to his team-mates ahead of the second leg at White Hart Lane later this week.
Disappointing result today. Gutted to be out the cup! Time to focus for Thursday night now! Big game for us!
— Harry Kane (@HKane) February 21, 2016
Tottenham moved to within two points of Leicester thanks to their impressive 2-1 victory over title rivals Manchester City at the Etihad Stadium in their most recent Premier League game.
Spurs host Swansea City in their next top-flight fixture in north London next Sunday.
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