The north London side lost 1-0 to Crystal Palace at White Hart Lane after former Liverpool defender Martin Kelly scored a first-half winner for the visitors.
Tottenham host Fiorentina in the second leg of their Europa League last-32 tie on Thursday night after a goalless draw in Florence last week.
Spurs have emerged as serious title contenders in recent weeks and Mauricio Pochettino’s side are two points behind leaders Leicester City.
England midfielder Alli is determined to ensure Spurs respond to Sunday’s setback with victory over Fiorentina later this week.
Alli wrote on Instagram: “We go again 💙#COYS”
Alli has scored seven goals and has made five assists in 24 Premier League games so far this season.
Tottenham have won their last five Premier League games ahead of the next top-flight fixture against Swansea City at White Hart Lane next Sunday.
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