The Red Devils will make the trip to Turin in mid-week after a 2-1 win over Bournemouth in the Premier League on Saturday afternoon.
Marcus Rashford scored an added-time winner to secure three points after Anthony Martial had cancelled out Callum Wilson’s opener.
Manchester United are unbeaten in their last four Premier League games, only dropping points in a 2-2 draw with title rivals Chelsea FC last month.
However, concerns remain about the fragility of Manchester United’s defence under Jose Mourinho after conceding 18 times in 11 league games this term.
Juventus eased to a 1-0 win over the Premier League side at Old Trafford last month after Paulo Dybala scored a first-half winner.
While the scoreline suggests their first meeting of the season was a tight game, Manchester United were thoroughly outplayed by the Serie A champions.
Former Arsenal playmaker Nicholas is tipping Juventus to ease to a 2-0 victory over Mourinho’s side at Old Trafford on Wednesday.
“I wasn’t overly impressed with Juventus in the first game, I don’t see why some people called them the best team in Europe – yes, the Cristiano Ronaldo addition was a good one but they didn’t blow me away,” Nicholas told Sky Sports.
“Paulo Dybala is a player I’ve always liked, and played well at Old Trafford and with him and Ronaldo around I genuinely don’t see Manchester United getting anything from this trip at all.”
Manchester United were 3-0 winners against Juventus in their last Champions League meeting in Turin when Ryan Giggs scored twice and Ruud van Nistelrooy found the net.
The Red Devils eased to a 3-2 victory over the Turin side in the Champions League semi-finals in 1999 when Roy Keane inspired a famous comeback win en route to winning the competition for the first time under Sir Alex Ferguson.
Cristiano Ronaldo failed to get on the score sheet at Old Trafford but the Portugal international will be hoping to inflict damage on his former club in Italy on Wednesday night.
The ex-Real Madrid star was a Manchester United player between 2003 and 2009, winning Premier League titles, the Champions League, the FA Cup and two English League Cups, scoring 118 goals in 292 appearances for the club.
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