Spurs lost 1-0 to Ajax in the first leg of the Champions League semi-final at the Tottenham Hotspur Stadium last week.
Donny van de Beek scored a first-half winner to put Ajax in control of the Champions League tie ahead of the return leg at the Amsterdam Arena.
Ajax are bidding to reach their first Champions League showpiece in 23 years, while Spurs have never reached the final of the competition.
Mauricio Pochettino’s side are on a disappointing run of form following three defeats in their last six games in the Premier League.
Former Liverpool FC striker Owen is backing Tottenham to get on the score-sheet in the Dutch capital but Ajax to progress to the Champions League final.
“Ajax have won at Real Madrid, Juventus and Spurs in the Champions League this season, but they have failed to win any of their last three home ties in the competition,” Owen told BetVictor.
“There are bound to be plenty of nerves on and off the pitch in Amsterdam and I expect Spurs to give the hosts plenty of problems despite their poor recent run of results.
“Spurs could, and probably should, have been out of sight by half-time at the Vitality Stadium on Saturday and it says much about the game when the Man of the Match award goes to the goalkeeper of the side who played the vast majority of the 2nd half with a two-man advantage.
“I expect to see Mauricio Pochettino’s side score in Holland, but I fear it will be the hosts who qualify for the Final in Madrid at the beginning of June.”
Tottenham were 3-1 winners against Ajax on their only previous trip to the Dutch capital in 1981 when goals from Glenn Hoddle, Mark Falco and Ricardo Villa sealed a resounding win.
Spurs lost 1-0 to Bournemouth at the Vitality Stadium on Saturday afternoon to drop down to fourth place in the Premier League table.
However, Pochettino’s side will still finish in the top four barring a disastrous heavy loss to Everton in their final fixture of the season, and Arsenal capitalising by claiming a big win at Burnley.
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